Constrained chromatin accessibility in PU.1-mutated agammaglobulinemia patients.

TitleConstrained chromatin accessibility in PU.1-mutated agammaglobulinemia patients.
Publication TypeJournal Article
Year of Publication2021
AuthorsLe Coz, C, Nguyen, DN, Su, C, Nolan, BE, Albrecht, AV, Xhani, S, Sun, D, Demaree, B, Pillarisetti, P, Khanna, C, Wright, F, Chen, PAmy, Yoon, S, Stiegler, AL, Maurer, K, Garifallou, JP, Rymaszewski, A, Kroft, SH, Olson, TS, Seif, AE, Wertheim, G, Grant, SFA, Vo, LT, Puck, JM, Sullivan, KE, Routes, JM, Zakharova, V, Shcherbina, A, Mukhina, A, Rudy, NL, Hurst, ACE, T Atkinson, P, Boggon, TJ, Hakonarson, H, Abate, AR, Hajjar, J, Nicholas, SK, Lupski, JR, Verbsky, J, Chinn, IK, Gonzalez, MV, Wells, AD, Marson, A, Poon, GMK, Romberg, N
JournalJ Exp Med
Date Published2021 Jul 05

The pioneer transcription factor (TF) PU.1 controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing nonpioneer TFs to enter otherwise inaccessible genomic sites. PU.1 deficiency fatally arrests lymphopoiesis and myelopoiesis in mice, but human congenital PU.1 disorders have not previously been described. We studied six unrelated agammaglobulinemic patients, each harboring a heterozygous mutation (four de novo, two unphased) of SPI1, the gene encoding PU.1. Affected patients lacked circulating B cells and possessed few conventional dendritic cells. Introducing disease-similar SPI1 mutations into human hematopoietic stem and progenitor cells impaired early in vitro B cell and myeloid cell differentiation. Patient SPI1 mutations encoded destabilized PU.1 proteins unable to nuclear localize or bind target DNA. In PU.1-haploinsufficient pro-B cell lines, euchromatin was less accessible to nonpioneer TFs critical for B cell development, and gene expression patterns associated with the pro- to pre-B cell transition were undermined. Our findings molecularly describe a novel form of agammaglobulinemia and underscore PU.1's critical, dose-dependent role as a hematopoietic euchromatin gatekeeper.

Alternate JournalJ Exp Med
PubMed ID33951726
PubMed Central IDPMC8105723
Grant ListUM1 HG006542 / HG / NHGRI NIH HHS / United States
R01 AI146026 / AI / NIAID NIH HHS / United States
T32 HD043021 / HD / NICHD NIH HHS / United States
K08 AI153767 / AI / NIAID NIH HHS / United States
P30 DK063720 / DK / NIDDK NIH HHS / United States