FAM92A Underlies Nonsyndromic Postaxial Polydactyly in Humans and an Abnormal Limb and Digit Skeletal Phenotype in Mice.

TitleFAM92A Underlies Nonsyndromic Postaxial Polydactyly in Humans and an Abnormal Limb and Digit Skeletal Phenotype in Mice.
Publication TypeJournal Article
Year of Publication2019
AuthorsSchrauwen, I, Giese, APj, Aziz, A, Lafont, DTino, Chakchouk, I, Santos-Cortez, RLyn P, Lee, K, Acharya, A, Khan, FSher, Ullah, A, Nickerson, DA, Bamshad, MJ, Ali, G, Riazuddin, S, Ansar, M, Ahmad, W, Ahmed, ZM, Leal, SM
JournalJ Bone Miner Res
Volume34
Issue2
Pagination375-386
Date Published2019 Feb
ISSN1523-4681
Abstract

Polydactyly is a common congenital anomaly of the hand and foot. Postaxial polydactyly (PAP) is characterized by one or more posterior or postaxial digits. In a Pakistani family with autosomal recessive nonsyndromic postaxial polydactyly type A (PAPA), we performed genomewide genotyping, linkage analysis, and exome and Sanger sequencing. Exome sequencing revealed a homozygous nonsense variant (c.478C>T, p.[Arg160*]) in the FAM92A gene within the mapped region on 8q21.13-q24.12 that segregated with the PAPA phenotype. We found that FAM92A is expressed in the developing mouse limb and E11.5 limb bud including the progress zone and the apical ectodermal ridge, where it strongly localizes at the cilia level, suggesting an important role in limb patterning. The identified variant leads to a loss of the FAM92A/Chibby1 complex that is crucial for ciliogenesis and impairs the recruitment and the colocalization of FAM92A with Chibby1 at the base of the cilia. In addition, we show that Fam92a homozygous mice also exhibit an abnormal digit morphology, including metatarsal osteomas and polysyndactyly, in addition to distinct abnormalities on the deltoid tuberosity of their humeri. In conclusion, we present a new nonsyndromic PAPA ciliopathy due to a loss-of-function variant in FAM92A. © 2018 American Society for Bone and Mineral Research.

DOI10.1002/jbmr.3594
Alternate JournalJ. Bone Miner. Res.
PubMed ID30395363
PubMed Central IDPMC6489482
Grant List(UM1HG006348) / / National Institute of Health /
(RO1DK114356) / GF / NIH HHS / United States
/ / Higher Education Commission, Islamabad, Pakistan /
UM1 HG006493 / HG / NHGRI NIH HHS / United States
R01DC016295 / GF / NIH HHS / United States
HG006493 / / National Human Genome Research Institute and the National Heart, Lung and Blood Institute /
R01 DC016295 / DC / NIDCD NIH HHS / United States