Whole-Exome Sequencing in Familial Parkinson Disease.

TitleWhole-Exome Sequencing in Familial Parkinson Disease.
Publication TypeJournal Article
Year of Publication2016
AuthorsFarlow, JL, Robak, LA, Hetrick, K, Bowling, K, Boerwinkle, E, Coban-Akdemir, ZH, Gambin, T, Gibbs, RA, Gu, S, Jain, P, Jankovic, J, Jhangiani, S, Kaw, K, Lai, D, Lin, H, Ling, H, Liu, Y, Lupski, JR, Muzny, D, Porter, P, Pugh, E, White, J, Doheny, K, Myers, RM, Shulman, JM, Foroud, T
JournalJAMA Neurol
Volume73
Issue1
Pagination68-75
Date Published2016 Jan
ISSN2168-6157
KeywordsAdult, Aged, Cohort Studies, Exome, Female, Genetic Predisposition to Disease, Genetic Variation, Humans, Male, Middle Aged, Parkinsonian Disorders, Protein-Tyrosine Kinases, Sequence Analysis, DNA, Tenascin
Abstract

IMPORTANCE: Parkinson disease (PD) is a progressive neurodegenerative disease for which susceptibility is linked to genetic and environmental risk factors.

OBJECTIVE: To identify genetic variants contributing to disease risk in familial PD.

DESIGN, SETTING, AND PARTICIPANTS: A 2-stage study design that included a discovery cohort of families with PD and a replication cohort of familial probands was used. In the discovery cohort, rare exonic variants that segregated in multiple affected individuals in a family and were predicted to be conserved or damaging were retained. Genes with retained variants were prioritized if expressed in the brain and located within PD-relevant pathways. Genes in which prioritized variants were observed in at least 4 families were selected as candidate genes for replication in the replication cohort. The setting was among individuals with familial PD enrolled from academic movement disorder specialty clinics across the United States. All participants had a family history of PD.

MAIN OUTCOMES AND MEASURES: Identification of genes containing rare, likely deleterious, genetic variants in individuals with familial PD using a 2-stage exome sequencing study design.

RESULTS: The 93 individuals from 32 families in the discovery cohort (49.5% [46 of 93] female) had a mean (SD) age at onset of 61.8 (10.0) years. The 49 individuals with familial PD in the replication cohort (32.6% [16 of 49] female) had a mean (SD) age at onset of 50.1 (15.7) years. Discovery cohort recruitment dates were 1999 to 2009, and replication cohort recruitment dates were 2003 to 2014. Data analysis dates were 2011 to 2015. Three genes containing a total of 13 rare and potentially damaging variants were prioritized in the discovery cohort. Two of these genes (TNK2 and TNR) also had rare variants that were predicted to be damaging in the replication cohort. All 9 variants identified in the 2 replicated genes in 12 families across the discovery and replication cohorts were confirmed via Sanger sequencing.

CONCLUSIONS AND RELEVANCE: TNK2 and TNR harbored rare, likely deleterious, variants in individuals having familial PD, with similar findings in an independent cohort. To our knowledge, these genes have not been previously associated with PD, although they have been linked to critical neuronal functions. Further studies are required to confirm a potential role for these genes in the pathogenesis of PD.

DOI10.1001/jamaneurol.2015.3266
Alternate JournalJAMA Neurol
PubMed ID26595808
PubMed Central IDPMC4946647
Grant ListHHSN268201100011I / / PHS HHS / United States
U54HG003273 / HG / NHGRI NIH HHS / United States
U54 HG006542 / HG / NHGRI NIH HHS / United States
T32 GM007526 / GM / NIGMS NIH HHS / United States
U54HG006542 / HG / NHGRI NIH HHS / United States
C06RR029965 / RR / NCRR NIH HHS / United States
HHSN268201100011I / HL / NHLBI NIH HHS / United States
HHSN268201100011C / HL / NHLBI NIH HHS / United States
TL1 TR000162 / TR / NCATS NIH HHS / United States
C06 RR029965 / RR / NCRR NIH HHS / United States
U54 HG003273 / HG / NHGRI NIH HHS / United States
UL1 TR001108 / TR / NCATS NIH HHS / United States
T32 GM07526-37 / GM / NIGMS NIH HHS / United States
R01NS037167 / NS / NINDS NIH HHS / United States
T32 GM077229 / GM / NIGMS NIH HHS / United States
R01 NS037167 / NS / NINDS NIH HHS / United States
R21 NS089854 / NS / NINDS NIH HHS / United States
1X01HG006236 / HG / NHGRI NIH HHS / United States
T32 GM008307 / GM / NIGMS NIH HHS / United States
R21NS089854 / NS / NINDS NIH HHS / United States